A mechanism for the indirect transfer of photosynthetically reduced nicotinamide adenine dinucleotide phosphate from chloroplasts to the cytoplasm.

نویسندگان

  • G J Kelly
  • M Gibbs
چکیده

A triose phosphate/3-phosphoglycerate shuttle for the indirect transfer of photosynthetically reduced NADP from chloroplasts to the cytoplasm has been demonstrated in vitro. Triose phosphate, formed from 3-phosphoglycerate in the chloroplast, was oxidized back to 3-phosphoglycerate outside the chloroplast by the nonreversible d-glyceraldehyde 3-phosphate dehydrogenase reaction which is specific for NADP. The 3-phosphoglycerate could presumably return to the chloroplast to complete the shuttle. The properties of nonreversible d-glyceraldehyde 3-phosphate dehydrogenase are considered particularly suitable for effective operation of this shuttle system.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Verifying of Participation of Nitric Oxide in Morphine Place Conditioning in the Rat Medial Septum Using Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase (NADPH-d)

Background: Role of nitric oxide (NO) in morphine-induced conditioned place preference (CPP) has already been proposed in the rat medial septum (MS), but no molecular evidence has been provided to clear this fact. Methods: Effects of intraseptal injections of L-arginine and/or NG-nitro-L-arginine methyl ester (L-NAME) on morphine place conditioning in Wistar rats were examined. Morphine (2.5-7....

متن کامل

REASSOCIATION AND REACTIVATION OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS AFTER DENATURATION BY 6 M UREA

Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

متن کامل

Interaction between ferredoxin and ferredoxin nicotinamide adenine dinucleotide phosphate reductase in pyridine nucleotide photoreduction and some partial reactions. I. Inhibition of ferredoxin nicotinamide adenine dinucleotide phosphate reductase by ferredoxin.

Purified ferredoxin has been shown to inhibit reactions mediated by the flavoprotein ferredoxin-NADP reductase. Ferredoxin inhibits the transfer of electrons from NADPH to ferricyanide (diaphorase activity) to NAD (transhydrogenase) and the photoreduction of pyridine nucleotides during the Hill reaction. On the basis of the kinetics of inhibition, it is suggested that the flavoprotein has two b...

متن کامل

Reduction of Trimethylene Dipyridyl with Illuminated Chloroplasts.

Chloroplasts photochemically reduce 1,1'-trimethylene-2,2'-dipyridylium dibromide and concurrently form adenosine triphosphate. Reduced trimethylene dipyridyl in darkness will reduce spinach ferredoxin, Clostridium pasteurianum ferredoxin, nicotinamide-adenine dinucleotide phosphate, and other viologen-type dyes.

متن کامل

N-Nitrosodimethylamine Carcinogenesis in Nicotinamide-deficient Rats1

Special diets that were extremely low in protein (5.5%) and high in carbohydrate were used to test the effect of nicotinamide on A/-nitrosodimethylamine-induced Carcinogenesis in Holtzman albino rats. The level of nicotinamide in the three diets ranged from 0 mg/kg of food to 50 mg/kg to 500 mg/kg. During the treatment with these diets (5 weeks) and the carcinogen (18 days), the renal and hepat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Plant physiology

دوره 52 6  شماره 

صفحات  -

تاریخ انتشار 1973